Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog

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Small Phage July - August Notebook: August 1 - August 16 Daily Log



Overview
March-April
May-June
July-August
September-October

8/1/13

- Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.


8/2/13

- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.

- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.

- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.


8/3/13

- E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Experiment 1.


8/4/13

- Started E coli liquid culture over night


7/26/13

- Performed second step of 8.2 Modeling Phage Plaque Sizes - Experiment 1


7/27/13

- Conducted 7.27 Bacteria Growth Curve After Dilution


7/28/13

- Started 15mL of BL21 liquid culture overnight.

- Started phage liquid culture to test for superinfection.


7/29/13

- Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol.

- Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)


7/31/13

- Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.


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