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From 2013.igem.org

Denaturing sonicated DNA

• Denaturing DNA by heat shock for 10mins in a 95C heat block
• Adding 5ul of OG dye

Running both denatured and sonicated samples on gel

• For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.
• 5ul of 1kb ladder was used for 3 wells each.

• The last 6 wells after ladder are the denatured DNA samples.
  • We were expecting to see fragmentation for the denatures samples.
  • There is no significant difference in between the sonicated samples and the sonicated and denatured ones.
  • There is a slight decrease in bands depending on the sonication time interval.
• Suggestions are to use ultra-sonification to view the effects with sonication focused just on sample, whereas the previous one was sonication water bath.