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Georgia State/28 May 2013
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Group C:
Digest 2μL linker B and C with EcoRI and NheI.
- Heat in activated for 20 minutes at 65°C.
Digest RFP samples 1-1, 2-1, 3-1 with BglII.
- Ran on gel at 70V for 90 minutes.
Digest pGAPzα B and C miniprep with EcoRI and XbaI.
- DNA precipitated resuspended in 50μL TE.
- Ran on gel at 70 V for 90 minutes.
Ran gel at 70V for 90 minutes with samples loading in the following order:
- 1kb + ladder, RFP 1-1, RFP 2-1, RFP 3-1, blank, pGAPzα B (3), blank, pGAPzα B (6), Blank, pGAPzα C (1), Blank, pGAPzα C (2).
Excised DNA from gel.
