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User contributions
From 2013.igem.org
- 17:15, 4 June 2013 (diff | hist) N Georgia State/30 May 2013 (Created page with "<u>'''5/30:'''</u><Br/> '''Group C:'''<Br/> Made LB + zeo plates.<Br/> Ligation of pGAPzα B and C from 5/29 and linkers digested 5/29.<Br/> Transformed E. coli.<Br/>") (top)
- 17:11, 4 June 2013 (diff | hist) N Georgia State/29 May 2013 (Created page with "<u>'''5/29:'''</u><Br/> '''Group C:'''<Br/> Finished gel extraction of digested pGAPzα B and C.<Br/> Ligation of pGAPzα B and C with linker pieces digested 5/28.<Br/> Transform...") (top)
- 17:00, 4 June 2013 (diff | hist) N Georgia State/28 May 2013 (Created page with "<u>'''5/28:'''</u><Br/> '''Group C:'''<Br/> Digest 2μL linker B and C with EcoRI and NheI.<Br/> <UL> <LI>Heat in activated for 20 minutes at 65°C.<Br/> </UL> Digest RFP samples...") (top)
- 15:39, 4 June 2013 (diff | hist) N Georgia State/22 May 2013 (Created page with "<u>'''5/22'''</u><Br/> -20°C freezer was left open overnight.<Br/> '''Group C'''<Br/> Gel showed DNA present in pGAPzα B and C digest.<Br/> Digest of linker with EcoRI and NheI...") (top)
- 15:22, 4 June 2013 (diff | hist) N Georgia State/21 May 2013 (Created page with "<u>'''5/21:</u>'''<Br/> '''Group A:'''<Br/> Made YPD broth.<Br/> '''Group B:'''<Br/> Miniprep of pGAPzα B.<Br/> '''Group C:'''<Br/> Miniprep of eight hour RFP culture from 5/20....") (top)
- 15:15, 4 June 2013 (diff | hist) N Georgia State/20 May 2013 (Created page with "<u>'''5/20:'''</u><Br/> '''Group A:'''<Br/> Overnight culture of pGAPzα A, B, C, and A modifid.<Br/> '''Group B:'''<Br/> Miniprep of pGAPzα B.<Br/> '''Group C:'''<Br/> Eight ho...") (top)
- 15:08, 4 June 2013 (diff | hist) N Georgia State/17 May 2013 (Created page with "'''5/7:'''<Br/> '''Group C:'''<Br/> Miniprep of 200 mL overnight culture from 5/16.<Br/> Glycerol stocks of E. coli + pGAPzα C.<Br/> Stored plates with P. Pastoris growth in ref...")
- 15:04, 4 June 2013 (diff | hist) N Georgia State/16 May 2013 (Created page with "'''5/16:'''<Br/> '''Group C:'''<Br/> Miniprep of overnight E. coli + pGAPzα C culture.<Br/> Restriction digest of miniprep samples 1-1, 2-1, 2-2 cut with either EcoRI or Bgl II....") (top)
- 16:44, 30 May 2013 (diff | hist) N Team:Georgia State/notebook (Created page with "Day 1 - 5/13 Team B: Made LB Broth and overnight cultures of pGAPzα B Team C: Made YPD broth; overnight cultures of pGAPzα C from plate 3; transformed E. coli cells with pGAPz...")
