"

From 2013.igem.org

• Home
• Team
  • Overview
  • Official Team Profile
  • Team Video
  • Undergraduates
  • Mentors
• Project
  • Overview
  • Background
  • Experimental Design
• Notebook
  • Overview
  • Phage Team
  • Cholera Team
• Results
  • Overview
  • Judging Criteria
  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
  • Overview
  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Contents 1 5/27/13 2 5/29/13 3 5/31/13 4 June 4.1 6/3/13 4.2 6/5/13 4.3 6/7/13

5/27/13

5/29/13

Today we wanted to see if we can transfer our current experiment from using E. coli W3110 as the host to E. coli B as the host. We set up multiple experiments.

1. We spot tested the following phage samples on W3110 and B: T1, T2, T3, T4Do stock, T4 mutated stock, T4-UV one-plaque plate harvest, T4-UV-mutated one-plaque plate harvest.
2. We did a dilution series on the T1 stock to see how concentrated it is. We will need to know the PFUs in order to grow a successful liquid culture and mass produce it for our large phage amplification procedure.
3. We also infected two samples of E. coli B, each with 10 uL of 10^-6 T4Do to observe plaque formation.

5/31/13

I took out the plates from last time's experiments.

1. The spot test showed that all seven samples cleared both E. coli B and W3110.
2. However, our dilution series for T1 did not work. All of the plates appeared to show only bacterial lawns. The plates were not observed after a 24 hour incubation, but 48 hours later there were only lawns on all the plates. 3. The 10^-6 T4Do plates produced excellent plaques, confirming that T4Do infects E. coli B.

Also, today Jade taught me how to set up the website- so I worked on that.

June

6/3/13

KS- I spent most of today getting the website in order and organized for future experiments to be entered in easily!

6/5/13

6/7/13