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Team:Newcastle/Notebook/calendar
From 2013.igem.org
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Contents |
6
10
Titles
- First Day of Training
Details
Morning
We started a discussion of our sub-project, doing some research into project presentation: wiki layout, t-shirt design and possible team logos. We looked at precedents for inspirational ideas.
Afternoon
Learn about Computational Modelling and BioBrick concepts. Decided to start with biophysical modelling of the lipid membrane of Bacillus subtilis and modelling of lipid synthetic pathway. Later in the day, we had a team meeting to discuss aims and progress of our work.
11
Modelling Training
- Modelling Training
- Laboratory Training
Details
Put any details you want here. Paragraphs... Lists... Images... Go nuts :)
6
13
Basic Lab Skills 1
- Basic Lab Skills 1
Details
Morning
On our first day of lab, we were briefed on various lab health and safety policies to ensure we maintained a safe working environment. We were then led by our supervisor on a tour around the lab to inform us about the layout. Once we have familiarised with everything, we officially started our first lab session.
We started by callibrating our pipettes and learning how to operate them, then we were shown how to innoculate bacteria to a liquid culture. Finally, before we took a break, we prepared bacteria streak plates.
Afternoon
In the afternoon, we extracted GFP and RFP bricks from the iGEM 2013 biobrick plate and transformed it into competent Escherichia coli cells. These procedures were performed using the following protocols (). Then plate those cells onto LB with chloramphenicol selection marker plates.
6
14
Basic Lab Skills 2
- Basic Lab Skills 2
Details
Morning
When we first arrived in the lab, we were all excited to check on our plates to see if anything grew. The RFP plates showed a lot of collonies, while the GFP plates did not work as expected. The next thing we did was too view the plates under the fluorescent inverted microscope.
Afternoon
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