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Team:BYU Provo/Notebook/Phage Purification/Springexp
From 2013.igem.org
| Phage Purification May - June Notebook
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May 1 - May 12
This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. During this week we began growing cultures to use and infect with phage to begin purification. We were held back waiting for materials, but were able to plan for our future procedures.
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| May 13 - May 26
In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome!
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| May 27 - June 9
We finished our CsCl gradient and have started a new round of phage purification to run through the CsCl gradient again.
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| June 10 - June 23
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| June 24 - June 30
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