20/09/13
From 2013.igem.org
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Miniprep from overnight broths
- DNA is isolated using Omega Miniprep kit and its protocol is followed
Nanodropping the isolated DNA
Sample | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
B5 |
Double digest of isolated DNA
- Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
- Gel to be run in the 23/09/13