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20/09/13
From 2013.igem.org
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Miniprep from overnight broths
- DNA is isolated using Omega Miniprep kit and its protocol is followed
Nanodropping the isolated DNA
| Sample | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| X12 | 90 | 13.5 | 1.86 | 1.38 |
| X3 | 43 | 13.6 | 2.12 | 1.34 |
| X5 | 45 | 16 | 2.08 | 1.64 |
| X6 | 44 | 19.1 | 2.04 | 1.59 |
| B4 | 93 | 11.1 | 1.73 | 1.28 |
| B5 | 87 | 9.5 | 1.99 | 1.34 |
| B9 | 43 | 13.1 | 2.14 | 1.43 |
| B12 | 43 | 14.9 | 1.99 | 1.23 |
| F1 | 92 | 17.6 | 1.91 | 1.15 |
| F8 | 93 | 17.6 | 1.85 | 1.70 |
| F10 | 91 | 12.4 | 1.99 | 1.30 |
| F12 | 41 | 18.5 | 2.06 | 1.71 |
Double digest of isolated DNA
- Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
- Gel to be run in the 23/09/13
