Team:USTC CHINA/Notebook/Protocols

From 2013.igem.org

Revision as of 13:00, 25 September 2013 by Ssq (Talk | contribs)

Gene Clone

Gel Extraction

DNA digestion

Single Digestion

Double Digestion

Colony PCR

PCR

Ligation

Plasmid mini-prep

Transformation

The transformation of E.coli

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measurement

Constitutive promoter measurements

plac promoter response to IPTG or Glucose

notebook

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols"