Team:USTC CHINA/Notebook/Protocols/PCR

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PCR

Performed with TaKaRa PrimeSTAR® GXL DNA Polymerase

Volume (μl)
5×PrimeSTAR GXL Buffer 10
dNTP Mixture(2.5 mM each) 4
Forward primer 10~15 pmol
Reverse primer 10~15 pmol
Template DNA 10pg~10ng
PrimeSTAR GXL DNA Polymerase 1
ddH2O To 50
Total 50

3. Gently vortex the samples and spin down.
4. Perform PCR using recommended thermal cycling conditions: