Team:HIT-Harbin/Parts

Revision as of 12:41, 26 September 2013 by Sema1688 (Talk | contribs)

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CODON OPTIMIZATION FOR HRPS AND HRPR GENE

hrpS gene sequence get from GeneBank

ATGACGATAAGGAATAGCAATGAATCCGTGCAGTCACTTCATTGTGCCTCCCATGAGTCCTCGTTAACGCATCCGTCGGAAGATATACACGGATCGCTATCTTCTCTCATCCACACTATCGCGCCCCTCAACGTTGATATCGTACTGGAAGGCGAAACCGGCACCGGCAAAGACACGCTGGCGCGCCGTATTCATCAGCTTTCCGGCTGCGCCGGCCCGTTGGTGGCGGTGAACTGCGCCGCCGTGCCGGAAACGCTGGCGGAAAGCGAGTTATTCGGGGTGGTGTCCGGCGCTTACACCGGCGCCAGCCGGTCCCGCGCCGGATACCTTGAAACCGCGGACAAAGGCATTCTGTTTCTGGATGAGATCGACAGTATGCCGCTGACGCTGCAGGCCAAAATGTTACGGGTTCTGGAAAGTCGCGGCATCGAACGGCTGGGCAGCACCCAATTCAAACCGGTGGATATGCGGGTGATTGTCGCCACGCAGACGCCATTGCAGAAACTGGTGGACGAAGGGCGTTTTCGGCGTGACCTCTACTTCCGGCTGGATACGGTAAAAATTCAGCTGCCGACGCTGCGTTCGCGTACGGAAATCATTCTGCCGTTATTCGAGCGCTTTTTGCTCGAGGCCTCGGCCCGCCTGAAACGGCCGGCGCCGGGCCTGTCGGCCATCATTCAGGAACAACTGCTGATGCACGGCTGGCCCGGCAACATTCGTGAGTTGAAGGCGGCGGCCGAGCGCTGGGTGCTGGGGCTGTCGCCGGTGCCGATAGCCAGTGATGGCGACGTGCAGGTAATGGGAGAGGATACGCCGCTGACGTCGCTGAAAGTGCGCCTGCGCCGCATTGAGCGGTTTCTGATTCAGGAAGCGTTGCAGCGCAACGACCACTGTATCGATACGGTGGTGAATGAACTGGGCATTCCCAAACGCACGCTCTATCACCGCATCAAGCTGCTGAATGTGGCGGCGCGCGGGCTGTAATAA

CAI-Value of the sequence before optimization:0.2735861795565913

GC-Content of the sequence before optimization:59.169199594731516

hrpS gene sequence after codon optimization

ATGACCATCCGTAACTCTAACGAATCTGTTCAGTCTCTGCACTGCGCTTCTCACGAATCTTCTCTGACCCACCCGTCTGAAGACATCCACGGTTCTCTGTCTTCTCTGATCCACACCATCGCTCCGCTGAACGTTGACATCGTTCTGGAAGGTGAAACCGGTACCGGTAAAGACACCCTGGCTCGTCGTATCCACCAGCTGTCTGGTTGCGCTGGTCCGCTGGTTGCTGTTAACTGCGCTGCTGTTCCGGAAACCCTGGCTGAATCTGAACTGTTCGGTGTTGTTTCTGGTGCTTACACCGGTGCTTCTCGTTCTCGTGCTGGTTACCTGGAAACCGCTGACAAAGGTATCCTGTTCCTGGACGAAATCGACTCTATGCCGCTGACCCTGCAAGCTAAAATGCTGCGTGTTCTGGAATCTCGTGGTATCGAACGTCTGGGTTCTACCCAGTTCAAACCGGTTGACATGCGTGTTATCGTTGCTACCCAGACCCCGCTGCAAAAACTGGTTGACGAAGGTCGTTTCCGTCGTGACCTGTACTTCCGTCTGGACACCGTTAAAATCCAGCTGCCGACCCTGCGTTCTCGTACCGAAATCATCCTGCCGCTGTTCGAACGTTTCCTGCTGGAAGCTTCTGCTCGTCTGAAACGTCCGGCTCCGGGTCTGTCTGCTATCATCCAGGAACAGCTGCTGATGCACGGTTGGCCGGGTAACATCCGTGAACTGAAAGCTGCTGCTGAACGTTGGGTTCTGGGTCTGTCTCCGGTTCCGATCGCTTCTGACGGTGACGTTCAGGTTATGGGTGAAGACACCCCGCTGACCTCTCTGAAAGTTCGTCTGCGTCGTATAGAGAGGTTCCTGATCCAGGAGGCTCTGCAACGTAACGACCACTGCATCGACACCGTTGTTAACGAACTGGGTATCCCGAAACGTACCCTGTACCACCGTATCAAACTGCTGAACGTTGCTGCTCGTGGTCTGTAATAA

CAI-Value of the optimized sequence:0.9381699423093808

GC-Content of the optimized sequence:53.596757852077005