Team:Yale/Project Export

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Revision as of 00:23, 27 September 2013 by EdKong (Talk | contribs)


Introduce type 1 secretion system to export and extract PLA

  • We needed a way to export the PLA once it was synthesized by the E. coli
    • We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli


  • Ms. Linton used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
    • This allowed the cells to export the PLA since the hlyA tag was attached to the granule
PHA export system.jpg



  • Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
PLAandPHAexport.png
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