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Experiment 1: Preparation of growth mediums (MRS and LB) (June 5th, 2013)

10 Agar LB plates

15 Agar MRS plates

Notes:

(1) 18.6g of Agar MRS were weighted to prepare 300ml of medium
(2) 7g of Agar LB were weighted to prepare 200ml of medium
(1) 5g of A-MRS were diluted in 80ml of distilled water
Another 5g and 170ml of distilled water were added
Small lumps were formed in the bottom of the Erlenmeyer flask
(2) 7g of Agar LB were distilled in 100ml of distilled water
Further 100ml of distilled water were added
It wasn’t possible to use a control of the sterilization because there was no autoclave tape
The autoclave began heating up
The autoclave valve was lowered
When the temperature of the autoclave reached 120⁰c, the power was cut to the half
The vapor cycle ended and the temperature decreased
The temperature reached 105⁰c and the valve was opened. The mediums were left inside the autoclave until de laminar flow cabinet was unoccupied
The medium in petri dishes were stored in the refrigerator
The growth mediums in storage were revised, they do not show signs of contamination

17 MRS Agar

9 LB Agar