Team:HIT-Harbin/Experiments
From 2013.igem.org
Project/experiments
Experiment Schedule
![](https://static.igem.org/mediawiki/2013/4/45/Experiment2.png)
Fig 1. PCR resuLts of our own parts
(1: hrpL; 2: hrpS; 3: hrpR; 4: Ptet +strong RBS+hrpS+T; 5:Ptet+intermediate RBS+hrpS+T; 6: Ptet+weak RBS+hrpS+T; 7: PIPTG +strong RBS+hrpR+T; 8 is not needed; 9: PIPTG +weak RBS+hrpR+T; 10: PhrpL+strong RBS+tetR+T; 11: PhrpL+intermediate RBS+tetR+T; 12: PhrpL+weak RBS+tetR+T; 13: PhrpL+strong RBS+RFP+T; 14: PhrpL+weak RBS+RFP+T; 15: PhrpL+weak RBS+RFP+T)
![](https://static.igem.org/mediawiki/2013/c/ca/Experiment3.png)
Fig 1.EcoR1 and Pst1 double restriction enzyme cleavage for hrpL AND gate(BBa_K1014014) device and B-POM1(BBa_K1014999)
1:plasmid carrying BBa_K1014014; 2:double restriction enzyme cleavage for 1; 3:plasmid carrying BBa_K1014999; 4:double restriction enzyme cleavage for 3
1.Test of device
Table 1 IPTG formula
![](https://static.igem.org/mediawiki/2013/a/a9/Experiment4.png)
![](https://static.igem.org/mediawiki/2013/c/c2/Experiment5.png)
Fig 3. No red after night
![](https://static.igem.org/mediawiki/2013/1/10/Experiment6.png)
Table 2 Dilution of Two groups of bacteria
![](https://static.igem.org/mediawiki/2013/2/21/Experiment7.png)