Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/5.5 Bacteria Cultures, 25 mL

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Phage Purification May - June Notebook: Experiments

Phage Purification

March-April

5.5 Bacteria Cultures, 25 mL

I) Purpose

Grow bacteria for later use in purification of phage.

II) Expected Outcome

Viable liquid cultures of E. coli, both W3110 and BL21

III) Reagants Used

W3110 and BL21 freezer stock

LB

IV) Actual Procedure

Infect 25 mL of LB with 1 loop-full of bacterial freezer stock and leave in shaker at 37^◦ overnight.

V) Results

Growth of BL21 liquid cultures of bacteria to infect and amplify amounts of phage. W3110 failed to grow, so the experiment will have to be repeated.