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From 2013.igem.org

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• iGEM 2013

Phage Purification August - September Notebook: September 1 - September 14 Experiment Listing

Phage Purification March-April May-June July-August September-October

9.7 CsCl Gradient This procedure purifies mutated T7 phage from the small phage team. Detailed procedure: 9.7 CsCl Gradient 9.9 10X Phage Suspension Buffer Creation We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients. Detailed procedure: 9.9 10X Phage Suspension Buffer Creation 9/11/13 - We ran another T7 phage purification procedure with new PSB. Detailed procedure: 9.11 CsCl Gradient