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From 2013.igem.org

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Results

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Plasmid extraction from synthetized parts

pUC57: 2710 bp

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp

• The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp

• The sample of the lane 7 is the RBS, inserted sequence: 76 bp

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Parts clonning in pSB1C3: 2070 bp

• In the lane 1 is the ladder of 3000 bp

• The sample of the lane 3 is the promoter 2

• The sample of the lane 4 is the promoter 1

• The sample of the lane 7 is the RBS

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Ligation Promoters with GFP

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the Plasmid GFP

• The sample of the lane 5 is the Plasmid GFP

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GFP expression for L.Plantarum promoter cloned in E.coli