NahR
Main Page-BBa_K1031610
Pre-existing Part: Main Page-BBa_J61051
BBa_K1031610 is upgraded from previous part BBa_J61051 designed by Arkin Lab. NahR is a 34 kDa transcription factor mined from Pseudomonas putida which binds to nah and sal promoters to activate transcription in response to the inducer salicylate. We constructed a Psal/NahR biosensor circuit using sfGFP as reporter gene and systematically characterized its performance . Adaptor of this biosensor not only improves detection efficiency but also expands detection profile.
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Parts for Biosensors

DmpR
BBa_K1031221 BBa_K1031222 BBa_K1031223 BBa_K1031224 is the DmpR biosensor library using different RBS preceding sfGFP.

HbpR
BBa_K1031300 is the coding sequence of HbpR with terminators.
BBa_K1031301 and BBa_K1031302 is the HbpR biosensor library using different RBS preceding sfGFP.

HcaR
BBa_K1031410 is the coding sequence of HcaR with terminators.
BBa_K1031441 BBa_K1031442 BBa_K1031443 and BBa_K1031444 are HcaR transcriptional factors with different constitutive promoters (Pc).
BBa_K1031420 and BBa_K1031421 are is the HcaR biosensor library using different RBS preceding sfGFP.

CapR
BBa_K1031100 is the coding sequence of CapR transcriptional factor.
BBa_K1031111 and BBa_K1031112 are basic sensor constructions with different CapR expression level.
BBa_K1031113 and BBa_K1031114 are reporters with different RBS for the CapR biosensor.

HpaR
BBa_K1031510 is the coding sequence of HpaR transcriptional factor.
BBa_K1031520 and BBa_K1031521 are reporters with different RBS for the HpaR biosensor.

NahR
BBa_K1031610 is the NahR biosensor circuit.

PaaX
BBa_K1031710 is the coding sequence of PaaX with terminators.
BBa_K1031742 BBa_K1031743 and BBa_K1031744 are PaaX transcriptional factors with different Pc.

XylS
BBa_K1031911 is the coding sequence of XylS transcriptional factor with terminators.
BBa_K1031912 BBa_K1031913 and BBa_K1031916 are XylS transcriptional factors with different Pc.

XylR
BBa_K1031803 BBa_K1031804 are XylR biosensor's reporters with different RBS.

Parts for Adaptors

NahF
BBa_K1031620 is the coding sequence of XylS transcriptional factor with terminators.
BBa_K1031621 BBa_K1031622 BBa_K1031623 BBa_K1031624 and BBa_K1031625 are coding sequences of enzyme NahF under different Pc.

Parts for Band-Pass Filter

BBa_K1031021 is the hybrid promoter which can be activated and inhibited at the same time by two different transcriptional factors.
BBa_K1031011 is the transcriptional factor used as the activator in the Band-pass Filter.
Each BBa_K1031013 BBa_K1031014 BBa_K1031015 BBa_K1031016 is cI with different of RBS and pSal as its promoter, which is a crucial part for band-pass filter.

Figure 1. The aromatics spectrum showing the aromatics-sensing profiles of our individual biosensors.
Each color segment in the central spectrum represents the detection profile of a biosensor. Structural formula highlighted in color stand for the aromatic compounds that can be detected by our biosensors.┝ stand for Adaptors, enzymes that convert undetectable compounds into detectable aromatics, thus to expand the detection capacity of some biosensors. Click Here for the summary table of the aromatics spectrum and aromatics-sensing profiles of individual biosensors.
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Figure 2. Experimental points and the linear fitting curves of the orthogonality test. The black dashed lines are with the slopes of 1, showing as the reference line. The slopes of the experimental fitting curves were showed in the upside portion of the figure, all of them were around 1. These data showed the orthogonality among inducers of biosensors(a, b) XylS and NahR; (c, d) XylS and HbpR; (e, f) NahR and HbpR, (g, h) XylS and DmpR, (i, j) NahR and DmpR, and (k, l) HbpR and DmpR. The experimental points and linear fitting curves of biosensor and its inducers are marked in different colors: XylS in red, NahR in green, HbpR in orange and DmpR in dark cyan.(For more details, please Click Here)

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