19 September 2013

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Lab 115

Main purpose

The purpose of this experiment is to measure nitric and nitrous oxide from the Nir transformant anaerobically while we spike it with nitrite.

Who was in the lab

Kashia, Ariadni

Procedure

This experiment is based on Experiment 2 for the Nir transformant, with some changes.

The experiment started with a temperature of around 37 _o C, initial OD of 0.1036 and initial volume of 200 ml. After resuspension the OD was really low around 0.0329.

The experiment starts, taking one sample and after 7 minutes, a second spike and then we spike with 2ml of nitrite and we take two samples. Afterwards we also spike with 2 ml of nitrite again and finally take one more sample.

Results

Colorimetric results

Ranges

Measuring range 5-150 mg/L NH₄-N
Measuring range 0.02-1 mg/L NO₂-N

salt (mg/L)	sample 1	sample 2	sample after spike	sample 4	sample 5
ammonium	<2	<2	<2	<2	<2
nitrite	<0.02	<0.02	0.74	1.24	1.34

Nir Transformed E. coli

Conclusion

The NO sensor was drifting and no proper results were obtained.