Team:UNITN-Trento/Project/Bacillus

Bacillus Subtilis When we first came up with the idea of B- fruity, we immediatly thought that b.subtilis was the perfect chassis for a possible marketable application:

1. Bacillus subtilis sporulates and it can be stored in a inactive state;
2. Bacillus subtilis is not pathogenic;
3. we have always worked only with E. coli and we thought to try out how it is to employ a new organism. It’s been really challenging but we got it!

Bacillus subtilis would be the perfect chassis for a fruit-ripening household product, that exploit ethylene (or MeSA) production upon spores activation. We have designed a B. fruity home edition that exploits this principle.
To achieve this goal we started working with EFE, a ethylene forming enzyme from Pseudomas Syringae pv. phaseolicola (BBa_K1065002), which were inserted into pSBBs0K-Pspac (IPGT inducible) and pSBBs4S-Pxyl (xylose inducible), two biobrick plasmids designed for B. subtilis by the iGEM 2012 LMU Munich team (please note that we used a new functional version of these plasmids, that were sent to us from LMU Munich).