Team:Dundee/Parts/Ourbiobricks

We have submitted two BioBricks to the Registry of Standard Biological Parts that will hopefully be of use to future teams and projects.

1. BBa_K1012001 Protein Phosphatase 1

Human Protein Phosphatase 1 (PP1) is a protein from the family of serine/threonine phosphatases, we have used it as a microcystin binding protein however it regulates many processes in the body therefore it may be used in many other ways.

2. BBa_K1012005 ompC-GFP reporter construct.

This is an improved version of BBa_R0083. BBa_R0083 comprises the ompC promoter, containing OmpR-binding sites. To improve this brick we added a strong Ribosome Binding Site (RBS; from BBa_B0034) followed by Green Fluorescent Protein (BBa_E0040). This was achieved by digesting BBa_R0083 with SpeI + PstI . The RBS from BBa_B0034 was excised with XbaI / PstI, and ligated into the BBa_R0083. The resultant plasmid was digested with SpeI + PstI , and was ligated with the GFP-encoding gene which had been excised from BBa_E0040 by digestion with XbaI / PstI. The resultant plasmid, Bba_ K1012005 responds to the osmotic activation of the EnvZ by producing green fluorescence. This part has been verified to work in this way.

Due to the time constraints we have been unable to submit the three BioBricks described below however, we hope to submit those once the registry re-opens again.

The signal sequence of MalE

Signal allowing for protein transport via the general secretory pathway (Sec) in E. coli

The signal sequence of PrsA

Signal allowing for protein transport by the general secretory pathway (Sec) in B. subtilis. This signal sequence also contains a lipobox, facilitating anchoring to the outer leaflet of the cytoplasmic membrane.

The signal sequence of TorA.

Signal allowing for protein transport via the E. coli Twin Arginine Translocation pathway (Tat) into the periplasm.