Team:Paris Bettencourt/Project/Overview
From 2013.igem.org
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To defeat tuberculosis, we need new biotechnology. Our work will add 4 new tools to the anti-TB medical armamentarium. Detect - A CRISPR-based biosensor delivered by phage and sequence-specific for antibiotic resistance. Target - An E. coli model hosting an essential mycobacterial metabolic pathway that could simplify drug screening. Infiltrate - A E. coli strain capable of entering infected macrophages and lysing mycobacteria. Sabotage - A non-lytic phage that spreads horizontally in a bacterial population and expresses an siRNA to knock down antibiotic resistance.
Target
![](https://static.igem.org/mediawiki/2013/1/11/PB_TargetIcon.gif)
test
Infiltrate
![](https://static.igem.org/mediawiki/2013/6/6c/PB_infiltrate.gif)
An effective TB therapy must reach mycobacteria inside lung macrophages. In this system, E. coli express lysteriolysin O (LLO) to enter the macrophage cytosol and Trehalose Dimycolate Hydrolase (TDMH) to degrade the pathogen's membrane.