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Team:Newcastle/Project/shape shifting
From 2013.igem.org
Contents |
Shape-shifting
Team Bio
Our Project
We aim to put L-forms into a single cell wide microfluidics channels (that are as small as 1 μm wide) with differently shaped end chambers. We will then observe whether the L-forms will grow and take up the shape of these chambers due to their lack of cell wall.
Microfluidics Microfluidics are a manipulation of fluid in the micro domain. The microfluidics chambers will be designed using autoCAD, software used for computer-aided design and drafting, to produce silicon wafer master moulds. Directed flow within the microfluidic wafer will physically manoeuvre L-form cells into the designed chambers, where they will be maintained by nutrient media. This will enable single cell level analysis for L-forms.
Visualization of Cell Membrane We would also be able to visualise the shape of the cell membrane and confirm our hypothesis by using FM.595 membrane stain to stain the membrane red. This will show that the shape of L-forms can be easily manipulated.
Abstract
Our aim is to shape the bacteria to fit different molds using the microfluidics system, model the biophysical characteristics of bacterial cell membrane, and see what happens when we switch the cell wall synthesis back on.
