Team:TU Darmstadt/materials/Buffers

From 2013.igem.org

Revision as of 20:13, 4 October 2013 by Janwin (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Labbook | Materials | Protocols

Buffers

Tris

Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C₄H₁₁NO₃, 2-Amino-2-hydroxymethyl-propane-1,3-diol).

1x PBS

8.18 g NaCl (140 mM)
0.2 g KCl (2.7 mM)
1.77 g Na₂HPO₄ (10 mM)
0.24 g KH₂PO₄ (1.8 mM)
Add. 1L dH₂O
Adjust to pH 7.4

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/materials/Buffers"