Team:Paris Bettencourt/Parts

We have submitted twelve new biobricks to the parts registry. Each subproject submitted parts so you can find e.g. the gRNA anti KAN from Detect, the SirA gene of M. Smegmatis of the Target team, the TDMH gene from Infiltrate and sRNA anti Cm from the Sabotage team.

We upgraded the standard biobrick shipping vector pSB1C3 with a new set of 4 ori’s and antibiotic resistance cassettes so that they can easily be co-expressed.The system of 4 backbones all have compatible origins and resistance cassettes that allows for up to 4 back bones to be expressed in a single host bacteria.

Here, we show a novel suggestion for a standard. This suggestion opens up the possibility of keeping the BioBrick standard and adds at the same time the needed tools to perform the assembly of several parts in one step. BBG is a fusion out of the BioBrick standard cloning and Gibson isothermal assembly.