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From 2013.igem.org

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Automatically determined parameters using the [http://www.mediawiki.org/wiki/Table BioBrick-AutoAnnotator]

BioBrick: BBa_K1159000

Used open reading frame from position ?x? to ?y?.

A (Ala)

10 (4.00%)

R (Arg)

8 (3.20%)

N (Asn)

13 (5.20%)

D (Asp)

18 (7.20%)

C (Cys)

2 (0.80%)

Q (Gln)

8 (3.20%)

E (Glu)

16 (6.40%)

G (Gly)

25 (10.00%)

H (His)

10 (4.00%)

K (Lys):

20 (8.00%)

M (Met)

6 (2.40%)

F (Phe)

13 (5.20%)

P (Pro)

11 (4.40%)

S (Ser)

9 (3.60%)

T (Thr)

16 (6.40%)

W (Trp)

1 (0.40%)

Y (Tyr)

12 (4.80%)

V (Val)

17 (6.80%)

Amino acid counting:

Total number of amino acids (aa):

250

Number of positively charged aa:

?9?

Number of negatively charged aa:

?9?

Biochemical parameters:

Molecular weight [Da]:

24190.279999999995

Theoretical pI:

?4?

Extinction coefficient:

?4?

Estimated half-life:

Mammals:

?4 h?

Yeast:

?4?

E. coli:

?4?

Codon usage:

Mammals:

?good?

Yeast:

?Ok?

E. coli:

?bad ?

RFC standard:

This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.

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