Team:TU Darmstadt/materials/Buffers

Tris

• Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C₄H₁₁NO₃, 2-Amino-2-hydroxymethyl-propane-1,3-diol).

1x PBS

• 8.18 g NaCl (140 mM)
• 0.2 g KCl (2.7 mM)
• 1.77 g Na₂HPO₄ (10 mM)
• 0.24 g KH₂PO₄ (1.8 mM)
• Add. 1L dH₂O
• Adjust to pH 7.4

TE buffer

• 10 mM Tris, bring to pH 8.0 with HCl
• 1 mM EDTA (Ethylenediaminetetraacetic acid)

50x TAE

• 242g Tris base in water
• add 57.1mL glacial acetic acid
• 100mL of 500mM EDTA (pH 8.0) solution
• add up to 1 litre of water