Exeter/10 July 2013
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Morning
MiniPrep of the following Parts:
- BBa_K322122, cyan pigment
- BBa_K592012, magenta pigment
- BBa_K592010, yellow pigment
Plasmids extracted are now in storage at -20oC.
Afternoon
Liquid cultures of the blue light module and magenta pigment
- BBa_K592010, yellow pigment
- BBa_K592012, magenta pigment
- BBa_B0015, a terminator
- BBa_K592005, FixJ protein intermediate
- BBa_K592004, blue light sensor
Retry of BBa_B0034 transformation
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
(Next day, results of BBa_B0034 transformation)
We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.