Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog

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Small Phage July - August Notebook: July 16 - July 31 Daily Log



Overview
March-April
May-June
July-August
September-October

7/16/13

- Started approximately 60mL of BL21 liquid culture overnight.


7/17/13

- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation

- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.


7/19/13

- Performed spot test for 7.17 Mutagen Concentration Test - Fifth Protocol

- Performed dilution series for


7/7/13

- Started 60mL of BL21 liquid culture overnight.


7/8/13

- Made 750mL of x8 top agar.

- We started our fourth round of mutagenesis in 7.8 Mutagen Concentration Test - Fourth Protocol


7/9/13

- Outreach @ Orem Public Library!

- Started approximately 20mL of E coli BL21 liquid culture overnight.


7/10/13

- Performed titer to determine mutated phage concentration for 7.8 Mutagen Concentration Test - Fourth Protocol


7/11/13

- Started approximately 10mL of E coli BL21 liquid culture overnight.


7/12/13

- Performed spot test to confirm the titer result from July 10th. Detail procedure listed in 7.8 Mutagen Concentration Test - Fourth Protocol.

- Started approximately 6 mL of E coli liquid culture overnight.


7/13/13

- Set up the phage liquid culture for propagation in 7.13 Phage Propagation Experiment.


7/14/13

- Started approximately 30mL of E coli BL21 liquid culture overnight.