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Team:UTK-Knoxville/5 August 2013
From 2013.igem.org
August 5th 2013
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Received primers for the TodS sensing domain from Brandon
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First attempted to PCR amplify the sensing domain from Psuedomonas Putida genomic DNA using a standard PCR protocol and a range of different possible annealing temperatures
- After all PCR reactions failed to produce any bands (detected via 1% agarose gel), moved to the first method outlined in the following paper.
- Since these reactions failed as well, August 6, 2013 will be spent using the BSA step PCR method with different organic solvents and in tandem with a touchdown pcr protocol
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