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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog
From 2013.igem.org
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8/1/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/2/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/3/13 - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1. - Discussed ideas for modeling phage plaque sizes
8/6/13 - Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1. - Started 5mL of E coli B liquid culture overnight.
8/7/13 - Performed phage titer determination for 8.2 Modeling Phage Plaque Sizes - Experiment 1.
7/29/13 - Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol. - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
7/31/13 - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.
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