{{:Team:Heidelberg/Templates/{{{2}}}}}

iGEM Main page

Here we are going to test our wiki design.

==Week 7== === (Konrad)=== ==== fragment amplification ==== ==> f1: (all) * 14.6 µl H2O * 25 µl Phusion Flash * 2x 0.5 µl Primer (NI01,NI06) * 0.4 µl template hasi {| class="wikitable" |- ! Cycles !! temperature [°C] !! Time [s] |- | 1 || 98 || 10 |- | rowspan="3"| 30 || 98 || 1 |- | 66 || 5 |- | 72 || 120 |- | 1 || 72 || 420 |- | 1 || 4 || inf |} [[File:Heidelberg teamfoto.jpg|400px|thumb|left|PCR for amplification of (all); lane 11]] :Gel purification (50µl): by accident did not take 3 but 1 Volume buffer QG :Labelling fragements: 1 I and 1 II (from different lanes)