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16/07/13
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Incubate in broth
- 4ml of luria broth in 6 universals
- Add 8ul of chloramphenicol
- Pick single colony and put in broth (sterile technique)
- Incubate overnight at 37 in shaker
Make agarose gel
- 20ml of 5xTBE
- 80ml of water
- 0.8g of agarose
(0.8% gel)
- boil till all agarose dissolved
- Cool
- Add 5ul of ethidium bromide
- Pour gel
Make buffer
- 50ml of 5xTBE
- 200ml of water
