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17/07/13
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Revision as of 11:07, 14 August 2013 by AliceHaworth (Talk | contribs)
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Isolating RFP plasmid
- Add 1.5ml of overnight culture to epindorph
- Centrifuge for 2mins
- Remove supernatent
- Repeat
- Use thermo scientific mini kit
- Measure concentration using nanodrop
Double digest
- 50ng of DNA
- 2ul of
