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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period4/Dailylog
From 2013.igem.org
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8/17/13 - Started characterizing post-CsCl phage in 8.14 Mutagen Concentration Test - Seventh Protocol. - Started approximately 10mL of E coli B liquid culture overnight. - Streaked out E coli B.
8/2/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/3/13 - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Discussed ideas for modeling phage plaque sizes
8/6/13 - Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Started 5mL of E coli B liquid culture overnight.
8/7/13 - Performed spot test to estimate phage titerfor 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Phage Purification Group started the purification process with CsCl gradient.
8/8/13 - Started approximately 15mL of E coli B liquid culture overnight.
8/9/13 - Performed Preliminary Titer for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/11/13 - Started approximately 20mL of E coli B liquid culture overnight.
8/12/13 - Performed titer - repeat for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments based on the results from 8.10 preliminary titer test. - Started T1 propagation. - Made accurate x2 top agar as preparation for 8.16 Modeling Phage Plaque Sizes - Experiment One.
8/13/13 - Started approximately 20mL of E coli B liquid culture overnight.
8/14/13 - Performed mutagenesis and spot test for 8.14 Mutagen Concentration Test - Seventh Protocol. - Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.
8/15/13 - T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 109 pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration. - Started approximately 30mL of E coli B liquid culture overnight.
8/16/13 - Started 8.16 Modeling Phage Plaque Sizes - Experiment One. - Phage Purification team performed the CsCl gradient for 8.14 Mutagen Concentration Test - Seventh Protocol.
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