"
19/08/13
From 2013.igem.org
Denaturing sonicated DNA
- Denaturing DNA by heat shock for 10mins in a 95C heat block
- Adding 5ul of OG dye
Running both denatured and sonicated samples on gel
- For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.
- 5ul of 1kb ladder was used for 3 wells each.
