Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Dailylog

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Small Phage May - June Notebook: May 27 - June 9 Daily Log



Overview
March-April
May-June
July-August
September-October

5/28/13

- Started three 8mL E coli BL21 liquid culture at around 4pm.


5/29/13

- Continued 5.20 Mutagen Concentration Experiment

- Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR

- Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. WE CAN START SITE DIRECTED MUTAGENESIS SOON!


5/15/13

- Completed 5.9 T7+ Liquid Culture Phage Concentration Test #2 by doing the spot test

- Performed 5.15 Titer Test on 5.3 T7 new Phage Stock to determine phage concentration and estimate dilution for applying mutagen

- Sorted LB plates made on May 8 and threw away the ones with obvious contamination


5/16/13

- Took plates from 5.15 out of incubation at around 4:00pm


5/17/13

- Determined that all LB plates from 5.8 had contamination

- Poured new LB plates

- Made x8 top agar


5/18/13

- Stacked up the LB plates made yesterday. No obvious sign of contamination seen.

- Threw away the 5.8 LB plates (the ones with contamination).


5/19/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7


5/20/13

- Performed T7 Mutagen Concentration Test

5.20 Mutagen Concentration Experiment

- Performed T7 Minor Capsid Protein PCR

5.20 T7 Minor Capsid Protein PCR


5/21/13

- Started two 5mL E coli BL21 overnight at around 7:00pm


5/22/13

- Performed spot test for 5.20 Mutagen Concentration Experiment

- Ran agarose gel to confirm PCR product

5.20 T7 Minor Capsid Protein PCR


5/23/13

- Started two 25mL E coli BL21 liquid culture over night at around 6:00pm


5/24/13

- Proceeded with 5.20 Mutagen Concentration Experiment by performing preliminary selection using x8 top agar


5/25/13

- Took pictures in preparation for Progress Report