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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Dailylog
From 2013.igem.org
| Small Phage May - June Notebook: May 27 - June 9 Daily Log
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5/28/13 - Started three 8mL E coli BL21 liquid culture at around 4pm.
5/29/13 - Continued 5.20 Mutagen Concentration Experiment - Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR - Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. WE CAN START SITE DIRECTED MUTAGENESIS SOON!
5/15/13 - Completed 5.9 T7+ Liquid Culture Phage Concentration Test #2 by doing the spot test - Performed 5.15 Titer Test on 5.3 T7 new Phage Stock to determine phage concentration and estimate dilution for applying mutagen - Sorted LB plates made on May 8 and threw away the ones with obvious contamination
5/16/13 - Took plates from 5.15 out of incubation at around 4:00pm
5/17/13 - Determined that all LB plates from 5.8 had contamination - Poured new LB plates - Made x8 top agar
5/18/13 - Stacked up the LB plates made yesterday. No obvious sign of contamination seen. - Threw away the 5.8 LB plates (the ones with contamination).
5/19/13 - Started two 5mL of E coli BL21 overnight - Designed procedure for applying mutagen and selecting for T7
5/20/13 - Performed T7 Mutagen Concentration Test - Performed T7 Minor Capsid Protein PCR
5/21/13 - Started two 5mL E coli BL21 overnight at around 7:00pm
5/22/13 - Performed spot test for 5.20 Mutagen Concentration Experiment - Ran agarose gel to confirm PCR product
5/23/13 - Started two 25mL E coli BL21 liquid culture over night at around 6:00pm
5/24/13 - Proceeded with 5.20 Mutagen Concentration Experiment by performing preliminary selection using x8 top agar
5/25/13 - Took pictures in preparation for Progress Report
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