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Team:Imperial College
From 2013.igem.org
We are engineering microbes to make environmentally friendly plastics from rubbish.
Europe's Newest Mountain, Mount Wasted
Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM for 48 hours.
Solid Recovered Fuel (SRF)
(A) WCM precursor material, this sterilised media made from LB and SRF was used to produce all WCM utilised. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 7 days growth in SRF.
(A) SRF in PBS (phosphate buffered saline), a buffer. We can see from this experiment whether our bacteria can grow solely on the waste SRF. (B) Cells containing mCherry pigment grown in SRF (A) over 3 days, then streaked in a qualitative assay to check for growth. (C) mCherry cells were streaked again after 6 days growth in SRF.
Cultured bacteria performing arts.
Project description
Waste is an inevitable product of the lives many of us wish to lead. From simple daily routines to essential activities such as agriculture, we produce huge amounts of material which needs somewhere to go or something to be done with. Our focus is on plastic waste due to its long life in the environment and the toxic byproducts of its degradation which negatively impact upon millions of people’s health and quality of life. It also poses unknown risks for the global ecosystem. The aim is to produce a biological cycle of plastic degradation and production - complete biological recycling. We are investigating how this will change the way we deal with plastic waste and importantly, the way we think about it.
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