"
15/07/13
From 2013.igem.org
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Count colonies on agar plates
| Plate | Colonies |
| Viability control | Covered |
| Negative control | 1 contaminant |
| 20ul of 50pg/ul | 135 |
| 20ul of 10pg/ul | 96 |
| 200ul of 50pg/ul | ~1600 |
| 200ul of 10pg/ul | 944 |
Therefore there was 1.2x10^8 cfu/ug
Replate to get individual colonies
- Take an individual colonies
- Streak out onto new plate
- Sterilize loop using bunsen burner
- Incubate at 37 overnight
