"

From 2013.igem.org

• Home
• Team
  • Overview
  • Official Team Profile
  • Team Video
  • Undergraduates
  • Mentors
• Project
  • Overview
  • Background
  • Experimental Design
• Notebook
  • Overview
  • Phage Team
  • Cholera Team
• Results
  • Overview
  • Judging Criteria
  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
  • Overview
  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Phage Purification March - April Notebook: Experiments

Phage Purification March-April May-June July-August September-October

4.2 W3110 50 mL Liquid Culture I) Purpose Grow bacteria for use in experiments. II) Expected Outcome Viable liquid culture of E. coli, W3110 III) Reagants Used W3110 plated colonies LB IV) Actual Procedure We put a colony from our W3110 plate of E.coli into 50mL LB broth and left in shaker at 37◦ overnight. V) Results Growth of W3110 liquid cultures of bacteria for use in phage amplification. The control tube showed no cantamination.