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17/07/13
From 2013.igem.org
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Isolating RFP plasmid
- Add 1.5ml of overnight culture to epindorph
- Centrifuge for 2mins
- Remove supernatent
- Repeat
- Use thermo scientific mini kit
- Measure concentration using nanodrop
Double digest
- 50ng of DNA
- 2ul of 3.1 buffer
- 1ul of each restriction (XbaI and PstIin the 6 samples, EcoRI and PstI in the 6 samples, 12 samples in total)
- Water to make total volume to 20ul
- Incubate at 60mins at 37
- 20mins at 80
