18/07/13

From 2013.igem.org

(Difference between revisions)
(Replaced content with "==Double digest==")
(→Double digest)
Line 1: Line 1:
-
==Double digest==
+
==Single digest==
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*Digesting RFP plasmid again using a single restriction enzyme- XbaI
 +
*Digesting DNA samples 1-6
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*200ng of DNA is run
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*Volumes for digest:
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{| border=1
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|Sample nr||1||2||3||4||5||6
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|-
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|DNA||0.7||0.7||0.8||1.3||1.2||2.6
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|-
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|Buffer||2||2||2||2||2||2
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|-
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|Water||16.3||16.3||16.2||15.7||15.8||14.4
 +
|-
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|XbaI||1||1||1||1||1||1
 +
|}

Revision as of 14:12, 22 August 2013

Single digest

  • Digesting RFP plasmid again using a single restriction enzyme- XbaI
  • Digesting DNA samples 1-6
  • 200ng of DNA is run
  • Volumes for digest:
Sample nr123456
DNA0.70.70.81.31.22.6
Buffer222222
Water16.316.316.215.715.814.4
XbaI111111
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