"
20/09/13
From 2013.igem.org
(Difference between revisions)
| Line 26: | Line 26: | ||
==Double digest of isolated DNA== | ==Double digest of isolated DNA== | ||
| - | *Digesting with EcorI-HF | + | *Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present. |
| - | + | *Gel to be run in the 23/09/13 | |
| - | + | ||
| - | *Gel | + | |
| - | + | ||
Revision as of 15:10, 23 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Miniprep from overnight broths
- DNA is isolated using Omega Miniprep kit and its protocol is followed
Nanodropping the isolated DNA
| Sample | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| B5 |
Double digest of isolated DNA
- Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
- Gel to be run in the 23/09/13
