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20/09/13
From 2013.igem.org
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(Created page with "==Miniprep from overnight broths== *DNA is isolated using Omega Miniprep kit and its protocol is followed ==Nanodropping the isolated DNA== {|border=1 |Sample||Volume ul||Concen...")
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(Created page with "==Miniprep from overnight broths== *DNA is isolated using Omega Miniprep kit and its protocol is followed ==Nanodropping the isolated DNA== {|border=1 |Sample||Volume ul||Concen...")
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Revision as of 10:03, 20 September 2013
Contents |
Miniprep from overnight broths
- DNA is isolated using Omega Miniprep kit and its protocol is followed
Nanodropping the isolated DNA
| Sample | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| B5 |
Double digest of isolated DNA
- Digesting with EcorI-HF and PstI-HF to see if insert has been incorporated into DNA
Running on a 1.5% gel
- Gel pre-made on 18/09/13
- Running at 100V for 1.5 hours
