"
29/08/13
From 2013.igem.org
(Difference between revisions)
(Created page with "==Make 2% agarose gel== ==Run Tod operon ligation genes on the gel== ==Isolate the DNA from gel== *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol") |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | <div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;"> | ||
| + | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
| + | !align="center"|[[Team:Leicester|Home]] | ||
| + | !align="center"|[[Team:Leicester/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
| + | !align="center"|[[Team:Leicester/Project|Project]] | ||
| + | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
| + | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
| + | |} | ||
| + | </div> | ||
==Make 2% agarose gel== | ==Make 2% agarose gel== | ||
==Run Tod operon ligation genes on the gel== | ==Run Tod operon ligation genes on the gel== | ||
| + | *1.5h at 70V | ||
| + | |||
==Isolate the DNA from gel== | ==Isolate the DNA from gel== | ||
*DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol | *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol | ||
| + | |||
| + | ==Measuring concentration of DNA using nanodrop== | ||
| + | {|border=1 | ||
| + | |Sample||Volume ul||Concentration ng/ul||260/280||260/230 | ||
| + | |- | ||
| + | |A1||30||14.7||1.81||1.41 | ||
| + | |- | ||
| + | |A2||31||13||1.71||1.43 | ||
| + | |- | ||
| + | |B1||30||23.6||1.93||1.02 | ||
| + | |- | ||
| + | |B2||29||21.9||1.88||1.33 | ||
| + | |- | ||
| + | |C1||29||14.7||1.83||0.63 | ||
| + | |- | ||
| + | |C2||30||15.5||1.86||0.64 | ||
| + | |} | ||
Latest revision as of 11:29, 2 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Make 2% agarose gel
Run Tod operon ligation genes on the gel
- 1.5h at 70V
Isolate the DNA from gel
- DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol
Measuring concentration of DNA using nanodrop
| Sample | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| A1 | 30 | 14.7 | 1.81 | 1.41 |
| A2 | 31 | 13 | 1.71 | 1.43 |
| B1 | 30 | 23.6 | 1.93 | 1.02 |
| B2 | 29 | 21.9 | 1.88 | 1.33 |
| C1 | 29 | 14.7 | 1.83 | 0.63 |
| C2 | 30 | 15.5 | 1.86 | 0.64 |
