Small Phage

From 2013.igem.org

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(May)
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==May==
==May==
 +
===5/1/13===
 +
 +
LP, XL
 +
 +
- Commencement of spring!!!
 +
- Discussed goals and outlined plans for spring term
 +
 +
===5/2/13===
 +
 +
LP, XL
 +
 +
- Designed primers for amplifying and sequencing phage capsid protein
 +
 +
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
 +
 +
===5/3/13===
 +
 +
LP, XL
 +
 +
- Performed agar test, focusing primarily on ×8
 +
 +
: [[5.3 T7 phage selection method test]]
 +
 +
- Processed phage amplification
 +
 +
: [[5.3 T7 phage amplification/purification]]
 +
 +
LP
 +
 +
- Started two 5mL overnights of BL21
===5/20/13===
===5/20/13===

Revision as of 18:19, 21 May 2013

Contents

March

April

May

5/1/13

LP, XL

- Commencement of spring!!! - Discussed goals and outlined plans for spring term

5/2/13

LP, XL

- Designed primers for amplifying and sequencing phage capsid protein

- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly

5/3/13

LP, XL

- Performed agar test, focusing primarily on ×8

5.3 T7 phage selection method test

- Processed phage amplification

5.3 T7 phage amplification/purification

LP

- Started two 5mL overnights of BL21

5/20/13

LP, XL

- We performed T7 Mutagen Concentration Test

5.20 Mutagen Concentration Experiment

- We performed T7 Minor Capsid Protein PCR

5.20 T7 Minor Capsid Protein PCR

June

July

August

September