User contributions
From 2013.igem.org
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- 18:40, 8 July 2013 (diff | hist) Team:NU Kazakhstan
- 18:29, 8 July 2013 (diff | hist) Team:NU Kazakhstan
- 18:28, 8 July 2013 (diff | hist) Team:NU Kazakhstan
- 10:42, 3 July 2013 (diff | hist) N 3 July 2013 (Created page with "<html> We have analyzed dsDNA sequences from SELEX cycles 5, 6, 7, 8 by running them through gel. However, no bands appeared, probably, because the concentration of the samples w...") (top)
- 15:53, 21 June 2013 (diff | hist) N 21 June 2013 (Created page with "<html> <div>We prepared LB agar/Ampicilin and LB agar/Kanamycin plates for later cloning procedure. Cloning is done on the last round of SELEX to select individual aptamers, whic...") (top)
- 14:36, 19 June 2013 (diff | hist) N 19 June 2013 (Created page with "<html> We continued doing SELEX 8 for CEA. We have done the following: <ol> <li>precipitation</li> <li>PCR</li> <li>Checked our PCR products by running them in the gel</li> </o...") (top)
- 14:21, 19 June 2013 (diff | hist) Team:NU Kazakhstan/Team
- 11:06, 19 June 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 11:04, 19 June 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 14:26, 17 June 2013 (diff | hist) N 17 June 2013 (Created page with "<html> <div>Today, we assisted Madina (one of our advisors) to do the SELEX cycle 8 (selecting aptamers against CEA) and to finish cycle 3 of SELEX (selecting aptamers against EC...") (top)
- 15:55, 14 June 2013 (diff | hist) N 14 June 2013 (Created page with "<html> We were planning to perform Western Blot analysis in order to check the specificity of binding between CEA protein and DNA aptamers from SELEX 7th and 8th cycles. However,...") (top)
- 13:38, 13 June 2013 (diff | hist) N 13 June 2013 (Created page with "<html> <div>We did PCR of the DNA aptamers from SELEX 8, and then two samples were run in the gel in order to check if their length is 80bp long.</div> <div>We also had a meeting...") (top)
- 12:47, 11 June 2013 (diff | hist) N 11 June 2013 (Created page with "<html> <div>1. Amplified the samples extracted from the yesterday's gel with PCR</div> <div>2. Runned the amplified dsDNAs on the gel (200V, 100mA)</div> <div>3. Observed the gel...") (top)
- 14:47, 10 June 2013 (diff | hist) N 10 June 2013 (Created page with "<html> <div>1. We decided to repeat Electric Mobility Shift Assay with different concentrations of CEA protein and DNA aptamers. We decided to take Protein:DNA ratio as 1:5, i.e....") (top)
- 14:16, 10 June 2013 (diff | hist) Team:NU Kazakhstan/Team
- 15:52, 7 June 2013 (diff | hist) Team:NU Kazakhstan
- 15:26, 7 June 2013 (diff | hist) Team:NU Kazakhstan
- 15:19, 7 June 2013 (diff | hist) N 7 June 2013 (Created page with "<html> <div>We had a meeting with our advisors, and today all members of the team were present (Alexander joined us today). Mukhtar made a presentation about what we did during t...") (top)
- 05:46, 7 June 2013 (diff | hist) Team:NU Kazakhstan/Team
- 05:42, 7 June 2013 (diff | hist) Team:NU Kazakhstan
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