Team:BYU Provo/Notebook/LargePhage/Springexp/Period3/Dailylog
From 2013.igem.org
(One intermediate revision not shown) | |||
Line 38: | Line 38: | ||
<font size="4"> '''5/29/13''' </font> | <font size="4"> '''5/29/13''' </font> | ||
+ | |||
Today we received our kit containing E. coli B, T1, T2, and T3. We streaked out some of the E. coli B on an LB plate in preparation to grow it in a flask and archive it in the freezer. | Today we received our kit containing E. coli B, T1, T2, and T3. We streaked out some of the E. coli B on an LB plate in preparation to grow it in a flask and archive it in the freezer. | ||
Line 50: | Line 51: | ||
<font size="4"> '''5/31/13''' </font> | <font size="4"> '''5/31/13''' </font> | ||
+ | |||
I took out the plates from last time's experiments. | I took out the plates from last time's experiments. | ||
+ | |||
+ | |||
[[File:ExampleA.jpg|center|400px]] | [[File:ExampleA.jpg|center|400px]] | ||
+ | |||
[[File:ExampleB.jpg|center|400px]] | [[File:ExampleB.jpg|center|400px]] | ||
+ | |||
<br>1. The spot test showed that all seven samples cleared both E. coli B and W3110. | <br>1. The spot test showed that all seven samples cleared both E. coli B and W3110. | ||
<br>2. However, our dilution series for T1 did not work. All of the plates appeared to show only bacterial lawns. The plates were not observed after a 24 hour incubation, but 48 hours later there were only lawns on all the plates. | <br>2. However, our dilution series for T1 did not work. All of the plates appeared to show only bacterial lawns. The plates were not observed after a 24 hour incubation, but 48 hours later there were only lawns on all the plates. | ||
Line 62: | Line 68: | ||
<font size="4"> '''6/3/13''' </font> | <font size="4"> '''6/3/13''' </font> | ||
+ | |||
KS- | KS- | ||
I spent most of today getting the website in order and organized for future experiments to be entered in easily! | I spent most of today getting the website in order and organized for future experiments to be entered in easily! | ||
Line 68: | Line 75: | ||
<font size="4"> '''6/5/13''' </font> | <font size="4"> '''6/5/13''' </font> | ||
+ | |||
Today we updated our experiments, daily log, and worked on our progress report. | Today we updated our experiments, daily log, and worked on our progress report. | ||
Latest revision as of 17:47, 27 September 2013
Large Phage May - June Notebook: May 27 - June 16 Daily Log
| ||
|
5/27/13 On Tuesday, the grids were imaged on the electron microscope. The film has not yet been developed, but the preliminary results were not what we were expecting in that we saw no giant phage in either sample. We will post images of the actual electron micrographs when they have been developed and scanned.
5/29/13 Today we received our kit containing E. coli B, T1, T2, and T3. We streaked out some of the E. coli B on an LB plate in preparation to grow it in a flask and archive it in the freezer. Today we wanted to see if we can transfer our current experiment from using E. coli W3110 as the host to E. coli B as the host. We set up multiple experiments.
5/31/13 I took out the plates from last time's experiments.
Also, today Jade taught me how to set up the website- so I worked on that.
6/3/13 KS- I spent most of today getting the website in order and organized for future experiments to be entered in easily!
6/5/13 Today we updated our experiments, daily log, and worked on our progress report.
|