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| - | ==May==
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| - | ===5/1/13===
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| - |
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| - | ===5/3/13===
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| - | ===5/6/13===
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| - |
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| - | ===5/8/13===
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| - |
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| - | ===5/10/13===
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| - |
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| - | ===5/13/13===
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| - |
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| - | ===5/15/13===
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| - |
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| - | ===5/17/13===
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| - |
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| - | ===5/20/13===
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| - | Today we need to run a dilution series to test the titer of our mutated phage stock.
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| - | We also need to start selecting for small plaques and learning how to pick them and titer them out.
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| - | We also should run a UV test on the mutated phage stock compared to the normal stock.
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| - | We picked one plaque off of the 180 sec UV plate sample. We suspended it in 1 mL of broth, and then UV-ed 20 uL samples at 45 second intervals. The number of plaques decreased the longer the samples sat under UV light. The samples were irradiated from 0 sec to 4 min 30 sec.
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| - | As a control, we diluted the T4Do stock to 10^-6 and tested 20 uL at 45 sec intervals (up to 6 min) under UV light.
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| - | Also, we diluted the T4 mutagenized stock to 10^-6 and tested 20 uL at 45 sec intervals (up to 4 min 30 sec) under UV light.
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| - | UV tests were done by placing 20 uL spots on parafilm and placed in a BSL-2 hood with the UV light turned on.
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| - |
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| - | ===5/22/13===
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| - | Results from 5/20/13 -
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| - | We left the plates in the incubator for 48 hours, which caused contamination on many plates to grow.
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| - | When checked at 24 hours, the T4 mutagenized stock had a web plate at 10^-3 and less than 5 plaques at 10^-6. This experiment will need to be redone from 10^0 down through 10^-6. The whole mutagenesis may need to be redone if this only represents a dilution of our titer when we were trying to grow it in liquid culture.
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| - | (We infected with ___ mL of phage at ___ titer in ____ vol of resuspended bacteria.)
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| - | Under UV light, the T4Do stock (diluted to 10^-6) has 19 plaques after being irradiated for six minutes (down from almost cleared at 0 min).
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| - | Under UV light, the 180 sec UV plate spot (diluted in 1 mL) has a few hundred plaques on it, but the amount dropped significantly at 4 min 30 sec from when it was UV-ed for 0 min.
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| - | We will re-titer the T4-Mut stock so we can learn whether it was diluted or whether an infection worked.
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| - | We will also re-run the UV test comparing the T4-Mut (10^-3) with the T4-Do stock (at 10^-6) for survivability. The mutagenized phage should survive better.
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| - | Since the loss of plaques seemed to level off for the T4Do stock at 5:15 (23 plaques) and 6 min (19 plaques), we will test a 7 min and 8 min timepoint to see if it stays level, suggesting these phage have multiple genomes and are severely mutated.
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| - | [[File:Apple.jpeg|600px|thumb|center|T4 mutant 0 and 1.5 minutes under UV light.]]
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| - | [[File:Blackberry1.jpeg|600px|thumb|center|T4 mutant 3 and 4.5 minutes under UV light.]]
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| - | [[File:Cranberry.jpeg|600px|thumb|center|T4 mutant 6 minutes under UV light.]]
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| - | [[File:Dingleberry.jpeg|600px|thumb|center|T4 mutant 7.5 minutes under UV light.]]
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| - | [[File:Eggplant.jpeg|600px|thumb|center|T4 mutant 9 minutes under UV light.]]
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| - | [[File:Eggplant.jpeg|600px|thumb|center|T4 mutant 0 and 1.5 minutes under UV light.]]
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| - |
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| - | ===5/24/13===
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| | ===5/27/13=== | | ===5/27/13=== |
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| | ===6/7/13=== | | ===6/7/13=== |
| - |
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| - | ===6/10/13===
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| - |
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| - | ===6/12/13===
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| - |
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| - | ===6/14/13===
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| - |
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| - | ===6/17/13===
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| - |
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| - | ===6/19/13===
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| - |
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| - | ===6/21/13===
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| - |
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| - | ===6/24/13===
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| - |
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| - | ===6/26/13===
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| - |
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| - | ===6/28/13===
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| - |
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| - | ==July==
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| - | ===7/1/13===
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| - |
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| - | ===7/3/13===
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| - |
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| - | ===7/5/13===
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| - |
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| - | ===7/8/13===
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| - |
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| - | ===7/10/13===
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| - |
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| - | ===7/12/13===
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| - |
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| - | ===7/15/13===
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| - |
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| - | ===7/17/13===
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| - |
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| - | ===7/19/13===
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| - |
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| - | ===7/22/13===
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| - |
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| - | ===7/24/13===
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| - |
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| - | ===7/26/13===
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| - |
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| - | ===7/29/13===
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| - |
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| - | ===7/31/13===
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| - |
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| - | ==August==
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| - | ===8/2/13===
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| - |
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| - | ===8/5/13===
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| - |
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| - | ===8/7/13===
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| - |
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| - | ===8/9/13===
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| - |
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| - | ===8/12/13===
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| - |
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| - | ===8/14/13===
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| - |
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| - | ===8/16/13===
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| - |
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| - | ===8/19/13===
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| - |
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| - | ===8/21/13===
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| - |
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| - | ===8/23/13===
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| - |
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| - | ===8/26/13===
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| - |
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| - | ===8/28/13===
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| - |
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| - | ===8/30/13===
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| - |
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| - | ==September==
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| - | ===9/2/13===
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| - |
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| - | ===9/4/13===
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| - |
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| - | ===9/6/13===
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| - |
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| - | ===9/9/13===
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| - |
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| - | ===9/11/13===
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| - |
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| - | ===9/13/13===
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| - |
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| - | ===9/16/13===
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| - |
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| - | ===9/18/13===
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| - |
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| - | ===9/20/13===
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| - |
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| - | ===9/23/13===
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| - |
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| - | ===9/25/13===
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| - |
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| - | ===9/27/13===
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| - |
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| - | ===9/30/13===
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| - | {{TeamBYUProvoFooter}}
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